Additional file 1 of Optimization of differential filtration-based mitochondrial isolation for mitochondrial transplant to cerebral organoids
Bibliographic record
Abstract
Additional file 1. Fig S1: Uncropped western blots from Fig. 1B of thymidine kinase and E1A in HEK293, r0, and post-mitochondrial transplant cells. Fig S2: Biological replicate of mitochondrial transplant of mitochondria isolated from HEK293 cell and transplanted to rho-0 (r0) cells. A) Assessment of ATP via cell titer glo with 48hr pretreatment of BrdU. B & C) Western blot of thymidine kinase and E1A in HEK293, r0, and post-mitochondrial transplant cells. Samples were prepared in laemmli buffer and loaded at 50μg/lane onto a 10% acrylamide gel and blotted for thymidine kinase (TK1; 1:1000) and HEK293 marker, E1A (1:1000). GAPDH (1:10000) was used as a loading control. Table S1: ΔCt of HEK293 whole cells and mitochondrial isolates for nDNA depletion and mtDNA enrichment, respectively.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.018 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.001 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.848 | 0.238 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".