A guide to selecting high-performing antibodies for Rab13 (UniProt ID: P51153) for use in western blot, immunoprecipitation, and immunofluorescence
Why this work is in the frame
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Bibliographic record
Abstract
This report presents a guide to selecting high-quality commercial antibodies against GTPase Rab13 in western blot using a standardized experimental protocol based on comparing read-outs in a knockout cell line and isogenic control; a knockdown cell line was also used to assess the capability of antibodies in immunoprecipitation and immunofluorescence. This work was supported by the CQDM (a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec) as well as the Government of Canada through Genome Canada, Genome Quebec, and Ontario Genomics (grant no. OGI-210).
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.006 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it