17:15 - 17:30: Elevated temperatures dampen innate immune responses of developing lake sturgeon (Acipenser fulvescens) when challenged with bacterial lipopolysaccharides
Bibliographic record
Abstract
Chronic temperature stress may leave freshwater fishes vulnerable to opportunistic pathogens, particularly during early life stages. Lake sturgeon, Acipenser fulvescens, populations within the northern expanse of their range in Manitoba, Canada, may be susceptible to high temperature thermal stress. We acclimated developing lake sturgeon for 21 days to two ecologically relevant temperatures (16 and 20oC) . Both acclimation treatments were exposed to 0, 30, and 60 µg.ml-1 bacterial lipopolysaccharides (endotoxins), as an immune stimulus, for 48 hr, with samples taken 4 and 48 hr during trial exposures and following a 7-day recovery period. We then measured whole body transcriptional (mRNA) and physiological (lysozyme activity, cortisol, glucose) responses involved in the innate immune, general stress, and fatty acid responses following acute exposure to bacterial endotoxins. Data detailing the responses involved in the above physiological processes revealed that while overall expression levels were higher at 20oC under control conditions, during bacterial stimulus 16oC reared lake sturgeon produced a more robust response with higher induced expression of the above responses than their 20oC acclimated counterparts. Additional whole-animal performance metrics (critical thermal maximum and metabolic rate) demonstrated acclimation-specific responses indicating compromised metabolic capacity following the initiation of immune related responses. Our study showed that acclimation to 20oC during early development impaired the activation of molecular pathways involved in the immune, stress, and fatty acid responses of lake sturgeon, highlighting the effects of chronic thermal stress on transcriptional activation and recruitment of downstream biological processes in this critically threatened and endangered species.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.000 |
| Bibliometrics | 0.004 | 0.003 |
| Science and technology studies | 0.001 | 0.003 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.004 | 0.002 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.032 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".