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Record W6931317372 · doi:10.5281/zenodo.7011213

CFIA-NCFAD/nf-flu v3.1.0

2022· other· en· W6931317372 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueZenodo (CERN European Organization for Nuclear Research) · 2022
Typeother
Languageen
FieldComputer Science
TopicMathematics, Computing, and Information Processing
Canadian institutionsCanadian Food Inspection Agency
Fundersnot available
KeywordsSequence (biology)Sample (material)DirectoryNanopore sequencingDebuggingReference genomeSequence assembly

Abstract

fetched live from OpenAlex

The workflow's name has been changed from <code>nf-iav-illumina</code> to <code>nf-flu</code> and the official repo for <code>nf-flu</code> will be CFIA-NCFAD/nf-flu going forward. Version 3 is a major release adding a Nanopore influenza sequence analysis subworkflow using IRMA for initial assembly and BLAST against NCBI Influenza DB sequences and optionally, user-specified sequences to identify the top reference sequence for each segment for each sample. A standard read mapping/variant calling analysis is performed: for each sample, Nanopore reads are mapped separately against each gene segment reference sequence using Minimap2; variant calling of read alignments is performed using Clair3; depth-masked consensus sequence is generated using Bcftools. Consensus sequences are BLAST searched against NCBI Influenza (and user-specified sequences) to generate a BLAST summary report and H/N subtyping report. MultiQC is used to summarize results into an interactive HTML report. NOTE: Read mapping/variant calling analysis has not been ported to the Illumina sequence analysis subworkflow. 3.1.0 changes Added back <code>bin/fastq_dir_to_samplesheet.py</code> for Illumina <code>--input</code> samplesheet creation from Illumina FASTQ reads directory Fixed issue #12. Nanopore sample sheet can specify a mix of single FASTQ files and/or directories containing FASTQ files. Different reads with the same sample name will be merged prior to analysis. FASTQs can be GZIP compressed and have the extensions: <code>.fastq</code>, <code>.fq</code>, <code>.fastq.gz</code>, <code>.fq.gz</code>. Updated CI tests to test for this flexible sample sheet handling. Switched to GitHub YAML form for bug report template from Markdown template. CI tests now output <code>results/pipeline_info/</code> and <code>.nextflow.log</code> as artifacts for easier debugging of issues.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Science and technology studies, Scholarly communication, Insufficient payload (model declined to judge)
Consensus categoriesInsufficient payload (model declined to judge)
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.133
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0020.000
Scholarly communication0.0020.000
Open science0.0040.004
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0500.017

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.028
GPT teacher head0.234
Teacher spread0.206 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it