Additional file 1 of Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis
Bibliographic record
Abstract
Additional file 1: Figure S1. Preparation of Arabidopsis thaliana for whole plant 13CO2 labeling experiments. A, Photosynthetically active surface area was calculated by photographing the rosette against a white background and comparison of the leaf surface to that of size standards, as determined by using the ‘Magic wand’ function in Adobe Photoshop CS5 to quantify pixels of each standard and leaf surface. B and C, examples of single plant labeling cuvettes with light and temperature control used in this study. The switch valve which alternates between ‘normal’ air and 13CO2-containing air is located just upstream of the cuvette inlet in both cases. Air is sampled by CO2 and H2O sensors before entering and after exiting the cuvette to calculate gas exchange parameters and confirm a photosynthetic steady state prior to switch to a 13CO2-containing atmosphere. D, a thermocouple in continuous contact with the abaxial leaf surface monitors leaf temperature during the acclimation and labeling phases of each experiment. Photo credit: M. Phillips.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.016 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.004 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.003 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.883 | 0.275 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".