Additional file 2 of IL-27 shapes the immune properties of human astrocytes and their impact on encountered human T lymphocytes
Bibliographic record
Abstract
Additional file 2. Supplementary Figure 2: IL-27-treated astrocytes enhance the expression of CD95, PD-L1 and ICAM-1 by T lymphocytes independently of residual IL-27 in the co-culture. A IL-27 levels measured in supernatants from untreated (white bar) or IL-27- exposed astrocytes (gray bar) and from astrocyte:CD4 T cell (pink) or astrocyte: CD8 T cell (green) co-cultures with astrocytes pretreated or not with IL-27. Each dot represents one sample. B Experimental procedure for T cell-astrocyte co-cultures. Human astrocytes were treated or not with IL-27 for 24h. Astrocytes were washed and then sIL-27Rα was added or not. One hour later, αCD3/αCD28 activated human CD4+ or CD8+ T lymphocytes were added to human astrocytes and co-cultured for 24h prior to being collected for flow cytometry analysis. C Percentage of positive T lymphocytes expressing CD95, PD-L1 and ICAM-1. n=3, 2 CD4+ T cell samples (white dots) and one CD8+ T cell sample (black dot).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.017 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.003 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.897 | 0.252 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".