Additional file 2 of TRIM25 promotes Capicua degradation independently of ERK in the absence of ATXN1L
Bibliographic record
Abstract
Additional file 2: Figure S2 ERK dysregulates CIC function. A) Representative Western blot of ATXN1LWT (NHA) and ATXN1LKO (B82) cell lines treated with FGF/EGF over 0-24 hours following serum starvation. FBS control was cultured in FBS for the duration of the timecourse. B) Representative Western blot of HOG cell line treated with FGF/EGF over 0-24 hours following serum starvation. FBS control was cultured in FBS for the duration of the timecourse. C) Representative Western blot of ATXN1LWT (NHA) and ATXN1LKO (B82) cell lines treated with FGF/EGF and/or MEK/ERK inhibitors trametinib/LY3214996. D) Relative mRNA expression of ATXN1L, CIC, and CIC target genes DUSP6, SPRY4, and ETV1/4/5 in ATXN1LWT (HEK) and ATXN1LKO (A30) cell lines treated with FGF/EGF for 8 hours following serum starvation. Gene expression was normalized to TBP and the serum starved parental ATXN1LWT (HEK) cell line was used as a relative control. E) Relative mRNA expression of ATXN1L, CIC, and CIC target genes DUSP6, SPRY4, and ETV1/4/5 in HOG cell line treated with FGF/EGF for 8 hours following serum starvation. Gene expression was normalized to TBP and the serum starved parental ATXN1LWT (HEK) cell line was used as a relative control. * RT-qPCR quantifications were collected from 3 independent experiments. Error bars represent one standard deviation. p-values were calculated using the two-tailed independent Student’s t-test. Statistically significant values are denoted (* = p < 0.05, ** = p < 0.01). Individual data values can be found in Additional file 17: Table S10.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.016 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.003 | 0.002 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.002 | 0.001 |
| Scholarly communication | 0.003 | 0.003 |
| Open science | 0.003 | 0.002 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.894 | 0.235 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".