<b>Bone marrow macrophage-exosomes with HuR deficiency limits ANG-II induced cardiac fibrosis and LV dysfunction in mice</b>
Bibliographic record
Abstract
Injection of diabetic (db/db) bone marrow macrophage-exosomes with HuR deficiency preserves LV function in ANG-II treated mice. Clodronate liposome or control liposome (150 μL; FormuMax Scientific, Sunnyvale, CA, USA) was intraperitoneally injected into 8-weeks old C57BL/6J mice and macrophage depletion was confirmed using FACS analysis (BD LSR II Analyzer). After 48 hours, vehicle (saline) or ANG II (1000 ng.kg−1.min−1; EMD Millipore) was continuously administered using osmotic mini-pumps (ALZET DURECT, Cupertino, CA). Exosomes from Control or HuR shRNA- treated BMMØ derived from diabetic (db/db) mice (Exo-db/dbHuR KD vs Exo-db/dbWT) were injected intravenous through tail vein on day 2 and day 4 after pump implantation. Heart function was evaluated by echocardiography (Vevo 2100; VisualSonics, Toronto, Canada). Mice were euthanized on day 28; heart was collected for further analysis. Transthoracic two-dimensional B-Mode and M-Mode echocardiogram was obtained using Vevo 2100 (VisualSonics, Toronto, Canada) equipped with 30 MHz transducer. Echocardiographic studies were performed after ANG II mini- osmotic pump implantation on mice anesthetized with a mixture of 1.5% isoflurane and oxygen (1 L/min). B-Mode and M-Mode tracings were used to measure left ventricle (LV) percent fractional shortening (%FS) and percent ejection fraction (�) respectively. Observation: Echocardiography analysis showing improvements in heart function as shown by higher % ejection fraction and % fractional shortening in mice receiving Exo- db/dbHuR KD. The full dataset is uploaded as an excel spreadsheet file.Masson’s Trichrome staining and analysis showing lower interstitial fibrosis in hearts of mice receiving diabetic (db/db) BMMØ-exosomes with HuR deficiency (Exo-db/dbHuR KD), as compared to db/db-Exo injected mice at 28 days post-administration. The full dataset is attached as a spreadsheet.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".