Histone acetyltransferase p300/CBP regulates reproductive diapause via the juvenile hormone pathway in the cabbage beetle, Colaphellus bowringi
Bibliographic record
Abstract
Juvenile hormone (JH) serves as a pivotal endocrine regulator of reproductive diapause in insects, though the molecular mechanisms governing JH signaling remain poorly characterized. This investigation assesses the roles of the histone acetyltransferase p300/CBP in mediating reproductive diapause through JH signaling in the cabbage beetle Colaphellus bowringi. RNA interference-mediated suppression of p300/CBP in reproductive female adults induced diapause-like phenotypes, including arrested ovarian maturation, suppressed vitellogenesis, enhanced lipid storage, and upregulated expression of diapause-associated genes. Along with downregulation of key JH biosynthetic enzymes and signaling components. While exogenous JH application decreased lipid accumulation, it failed to rescue ovarian developmental arrest. Parallel experiments in diapausing females with p300/CBP knockdown yielded similar results - JH analog treatment reduced lipid reserves without stimulating ovarian development. These findings collectively demonstrate that p300/CBP regulates photoperiodic reproductive diapause through dual regulation of JH biosynthesis and signal transduction pathways in C. bowringi. Our results establish p300/CBP as a critical mediator of JH biosynthesis and signaling during photoperiod-induced reproductive diapause. This work advances our understanding of epigenetic regulation in insect diapause and provides a molecular framework for future studies on environmental adaptation strategies in insects.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.003 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.003 | 0.005 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.024 | 0.010 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".