Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil
Bibliographic record
Abstract
A new actinomycete strain designated PP14 was isolated from a Canadian soil sample, by a dilution agar plating method using a chitin-vitamins B medium supplemented with different antibiotics as selective agents. This actinomycete produced antimicrobial substances and exhibited strong antifungal activity against mycotoxigenic fungi includingAspergillus carbonarius (M333) and Penicillium expansum, and also against phytopathogenic fungi such as Fusarium oxysporum f. sp. lini (Foln 3-5) and F. culmorum. In addition, the strain showed strong activity against the yeast Saccharomyces cerevisiae (ATCC 4226). On the other hand, the strain exhibited an interesting antibacterial activity against bacteria including multi-resistant Klebsiella pneumoniae(E40). The highest antimicrobial activities were obtained on ISP2 medium. The n-butanol extract contained three bioactive spots detected on thin layer chromatography (TLC) plates. Strain PP14 was identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Streptomyces. The 16S rRNA gene sequence similarities showed that strain PP14 is closely associated with members of the Streptomyces violaceoruberspecies group (S. violaceoruber, Streptomyces violaceolatus, Streptomyces tricolor,Streptomyces humiferus, Streptomyces coelescens and Streptomyces anthocyanicus). Furthermore, the comparison of physiological characteristics of strain PP14 with the S. violaceoruber species group showed significant differences. Our results showed that strain PP14 represents a distinct phyletic line suggesting a new genomic species. Key words: Actinomycetes, Streptomyces, antimicrobial compounds, extremophile microorganisms, multi-resistant bacteria, Canadian soil.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".