Additional file 18 of Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada
Bibliographic record
Abstract
Additional file 18: Fig. S9. SsCHC is distributed in the cytosol and interacts with a putative light chain structural analogue. (A) SsCHC was tagged C-terminally with three HA tags and immune-localized to the cellcytosol. Signal was observed in 88% of the analyzed cells (N = 171). (B) High resolution imaging of SsCHC using confocal imaging reveals CHC foci. (C) Single native co-IP analysis of HA-tagged SsCHC reporter (predicted at ca. 210 kDa) including distribution of the 171 proteins found in higher abundance with respect to a control co-IP experiment using extracts of non-transgenic Ss cells. (D) Qualitative immunoblot analysis of samples from the single native co-IP of HA-tagged SsCHC reporter. I: soluble native co-IP input; P: insoluble cell debris post cell lysis; F: native co-IP flow-through; B: anti-HA beads. WT: non-transgenic Ss cells. MW: molecular weight. (E) Ab initio in silico protein modelling with AlphaFold of Ss11905, GlACLC, TbCLC and HsCLC. TM-align and RMSD scores for predicted structures of Giardia ACLC, Trypanosoma brucei CLC and Ss11905 with respect to Homo sapiens CLC show overall structural conservation with respect to a bona fide CLC. Scale bars: (A) 20 μm. (B) 5 μm.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.021 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.003 | 0.004 |
| Science and technology studies | 0.002 | 0.001 |
| Scholarly communication | 0.003 | 0.003 |
| Open science | 0.003 | 0.002 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.841 | 0.195 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".