Additional file 4 of Gut commensal Enterocloster species host inoviruses that are secreted in vitro and in vivo
Bibliographic record
Abstract
Additional file 4: Figure S4. Inoviruses are secreted in vitro. A Schematic demonstrating how inoviruses are detected in vitro. First, three-day-old cultures were separated to yield cell-free spent media supernatants that were filter-sterilized and then treated with DNase to eliminate contaminating bacterial DNA. The untreated (and unfiltered) and DNase-treated supernatants were then used as template DNA in qPCR and PCR reactions using primers targeting the pI gene and 16S rRNA gene to guarantee that there was no 16S contamination. qPCR assay using B pI and C 16S rRNA gene-specific primers in untreated and treated supernatants of Enterocloster cultures. Data shown for three biological replicates. Gel electrophoresis of PCR products from untreated and treated supernatants and D pI and E) 16S rRNA gene-specific primers.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.026 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.003 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.906 | 0.242 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".