Rapid Catalyst-Free Hydrazone\nLigation: Protein-Pyridoxal\nPhosphoramides
Bibliographic record
Abstract
Pyridoxal-5′-phosphate (PLP) represents an active\nform of\nVitamin B<sub>6</sub> that shows relatively fast imine formation with\nhydrazines under physiological conditions without the need of a catalyst.\nA convenient phosphate/amine conjugation protocol was developed to\ncovalently link PLP to proteins, affording proteins capable of hydrazone\nformation with bioorthogonal hydrazinyl functional groups. Thus, the\nlectin Concanavalin A (Con A) was labeled with PLP. Pretreatment with\nfluorescein hydrazide gave dye-labeled Con A that labeled cell surfaces\nefficiently. Alternatively, pretargeting was achieved by labeling\ncells with Con A-PLP, then treatment in vitro with Alexa Fluor 488\nhydrazide.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.423 | 0.004 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".