Molecular epidemiology of CM associated coliform bacteria isolated over a one year period from 11 dairy herds in Newfoundland.
Bibliographic record
Abstract
<p>(<b>A</b>) Results from RAPD analysis employing 1.5% agarose gels to separate PCR products. Chromosomal DNA from a laboratory strain (<i>K. pneumoniae</i> ATCC 15380) was included as the positive control and sterile water functioned as negative control in the PCR assays. A 100 bp ladder (Cat No. DM001-R500, GeneDirex, USA) was used as the molecular weight marker (<b>B</b>) Dendrogram based on RAPD results showing relatedness between strains subjected to analysis in the current study. The dendrogram was generated by the neighbor joining method with the PyElph software. (<b>A, B and C</b>) The isolates were assigned labels based on the farm or origin (F1 to F11) followed by a number to identify the infected animal. The isolates determined to be <i>E. cloacae</i> (*) and <i>K. variicola</i> (<sup>▴</sup>) are also indicated. (<b>B</b>) The results from antibiotic susceptibility testing are also shown next to each isolate. S: sensitive, I: intermediate and R: resistant designations are assigned based on established break points. The S/I/R designations for each drug are in the following order: cephalothin, ceftiofur, streptomycin, tetracycline and trimethoprim sulfamethoxazole, respectively. The isolates identified as <i>K. variicola</i> and <i>E. cloacae</i> on further analysis are also indicated. (<b>C</b>) Re-analysis of strains by RAPD PCR to verify the accuracy of the assay and to confirm identities. PCR products generated independently for the second time using DNA from identical strains along with the positive control were reanalyzed, which confirmed results shown in <b>A</b>.</p>
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.161 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".