Inhaled nitric oxide protects against hyperoxia-induced apoptosis in the rat lung.
Bibliographic record
Abstract
Inhaled nitric oxide (NO) is frequently administered in combination with hyperoxic gas mixtures to effect pulmonary vasodilation in patients with pulmonary hypertension. NO was recently shown to have a protective effect against the injurious consequences of prolonged hyperoxia. The present study investigated the possibility that this protective effect may be attributable to the ability of NO to block apoptosis. Rats exposed to 100% O2 for 60 h developed severe lung injury consisting of pronounced vascular leak and alveolar apoptosis, as inferred from the presence of DNA fragmentation (positive ISEL staining and DNA ladders in agarose gels), and a decrease in constitutive pro-caspase-3 levels. However, the inclusion of NO (20 ppm) in the hyperoxic gas mixture significantly attenuated both the vascular leak and apoptosis. NO reversed the hyperoxia-associated changes in the activity of the redox-sensitive transcription factors NF-kappaB, AP-1 and Sp1 after 24 h. This protective effect was accompanied by a decrease in the level of the proinflammatory protein ICAM-1 and an increase in the cellular antioxidant glutathione. This study shows for the first time that NO can protect against both hyperoxia-induced apoptosis and vascular leak. The data suggest that the protection may occur at the transcriptional and caspase activation level.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".