Additional file 1 of A new, reliable, and high-throughput strategy to screen bacteria for antagonistic activity against Staphylococcus aureus
Bibliographic record
Abstract
Additional file 1: Figure S1. Expression of the reporter protein in agr defective strain (RN4220) and positive strain (Sa25). Two plasmids pQS1 and pQS3 were transformed to S. aureus RN4220 (agr-) and Sa25. After 24 h of incubation either with or without trimethoprim, no fluorescent colony was observed from the transformants of S. aureus RN4220, while the transformants of S. aureus Sa25 exhibited clear fluorescent phenotypes. Figure S2. Agarose gel electrophoresis of plasmid DNA. The first lane contains a single DNA band of pCC97-1. Other lands contain two DNA bands corresponding to pCC97-1 and pQS plasmids, indicating the co-existence of two plasmids in the same S. aureus cells.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.023 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.868 | 0.214 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".