Additional file 5 of Channel nuclear pore protein 54 directs sexual differentiation and neuronal wiring of female reproductive behaviors in Drosophila
Bibliographic record
Abstract
Additional file 5 NUP54 is highly conserved, but variation in the FG repeat region is not the cause of an altered post-mating response. A) Sequence alignment of NUP54 from closely related species. Amino acids deviating from D. melanogaster are indicated in black. Intron positions are indicated by black arrowheads and the stop codon of the Nup54MB03363 allele is indicated by a red arrow head. Green and white filled arrowheads indicate maintained (>1%) and rare (<1%) polymorphisms with amino acid changes indicated on top. The line below the sequence indicates the NgoMIV-BamHI fragment that was replaced in the gNup54elegans construct. Nucleotides 1-115 according to human NUP54 impasse the FG region, nucleotides 116-346 the α/β region and nucleotides 346-494 the α-helical region. The amino acids in exon 3 have been shown to bind to NUP62 and the amino acids in exon 5 bind to NUP58. B) Receptivity of wild type, gNup54 and gNup54elegans females homozygous for Df(2R)9B4 after sex-peptide (SP) or Ringer’s (R) injection measured by counting mating females in a 1 hr time period 3 hr after SP or R injection, respectively. Means with the standard error for three experiments with 8-15 females each are shown, and statistically significant differences from ANOVA post-hoc pairwise comparisons are indicated by different letters (p≤0.001). C) Oviposition of gNup54 and gNup54elegans females homozygous for Df(2R)9B4 after sex-peptide (SP) or Ringer’s (R) injection shown as means of eggs laid in 18 h with the standard error for 10 females each, respectively. Statistically significant differences from ANOVA post-hoc pairwise comparisons are indicated by different letters (p≤0.001). The data underlying the presented graphs are in Additional file 6.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.015 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.867 | 0.184 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".