Additional file 1 of Channel nuclear pore protein 54 directs sexual differentiation and neuronal wiring of female reproductive behaviors in Drosophila
Bibliographic record
Abstract
Additional file 1 Nup54 is required before neuronal maturation for establishing the post-mating response. A) Receptivity of control (white for Ringer and black for SP injection) and transheterozygous Nup54MB03363/Df(2R)9B4 expressing UASNup54::HA with tubGAL4 (blue) ubiquitously, in neurons with elavGAL4C155 (purple) or glia with repoGAL4 (yellow), and in ppkGAL4 (orange), fruGAL4 (green) and dsxGAL4 (red) patterns after sex-peptide (SP, dark color) or Ringer’s (R, light color) injection measured by counting mating females in a 1 h time period 3 h after SP or R injection, respectively. Means with the standard error for three experiments with 15-21 females each are shown, and statistically significant differences from ANOVA post-hoc pairwise comparisons are indicated by different letters (p≤0.05). B) Oviposition of control (white for Ringer and black for SP injection) and transheterozygous Nup54MB03363/Df(2R)9B4 expressing UASNup54::HA with tubGAL4 (blue) ubiquitously, in neurons with elavGAL4C155 (purple) or glia with repoGAL4 (yellow), and in ppkGAL4 (orange), fruGAL4 (green) and dsxGAL4 (red) patterns after sex-peptide (SP, dark color) or Ringer’s (R, light color) injection shown as means of eggs laid in 18 h with the standard error for 10-15 females each, respectively, and statistically significant differences from ANOVA post-hoc pairwise comparisons are indicated by different letters (a, b: p≤0.001, c: ns). The data underlying the presented graphs are in Additional file 6.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.014 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.004 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.852 | 0.193 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".