Additional file 7 of Purine salvage promotes treatment resistance in H3K27M-mutant diffuse midline glioma
Bibliographic record
Abstract
Additional file 7: Supplemental Figure 5. Stable isotope tracing of de novo and salvage purine synthesis. A.) and B.) Schematics for (A.) 15N-Gln tracing and (B.) 2D-Hpx and the respective labeling pattern of each purine metabolite. Schematics were created using BioRender.com. C.) and D.) Percent of tracer metabolite enrichment in (C.)15N-Gln and (D.) 2D-Hpx pools in unirradiated DIPGXIII H3K27M-isogenic cells. E.) FC in relative 15N-labeled GMP(left) and AMP (right) abundance between irradiated H3K27M-isogenic cell lines. Data are normalized to H3K27M-KO samples. F.) FC in relative deuterium-labeled GMP (left) andAMP (right) abundance between irradiated H3K27M-isogenic cell lines. Data are normalized to H3K27M-KO samples. Statistical analyses were performed using two-tailed t-tests in GraphPad Prism 10.0.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.019 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.881 | 0.198 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".