Detection and identification of a phytoplasma affecting black currant and red currant shrubs in north America
Bibliographic record
Abstract
During October-November/2011, blackcurrant (Ribes nigrum L.) and\nredcurrant (Ribes rubrum L.) shrubs from Ontario, Canada that exhibited symptoms of\nleaf redness and downward curling were tested to verify phytoplasma presence. Total\nDNA was extracted and used in a nested PCR assay with universal primers that target\nthe phytoplasma 16S rRNA gene in nested reaction. Amplicons were obtained for all\nthe symptomatic plants collected and phytoplasma sequence from both blackcurrant\nand redcurrant plants showed a 99% sequence identity to those of members of group\n16SrX, subgroup 16SrX-A. Virtual RFLP of the R16F2n/R2 amplicons yielded\npatterns identical to those of ‘Ca. P. mali’ (AJ542541). Phylogenetic analysis based on\nthe 16S rDNA sequences confirmed RFLP results. Phytoplasmas of group 16SrX have\nbeen previously reported in Ontario, affecting Prunus and Pyrus species. However, our\nresults represent the first record of a 16SrX-A infecting blackcurrant and redcurrant in\nCanada and North America, and provide a valuable tool for further epidemiological\nstudies of a 16SrX phytoplasma in these two plant species.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".