Nucleotide sequence and phylogeny of a plastocyanin gene in the marine diatom, «Thalassiosira oceanica»
Bibliographic record
Abstract
Diatoms are thought to have acquired an Fe-containing cytochrome (cyt) c6 to transfer electrons between cyt b6f and photosystem (PS) I of the photosynthetic apparatus like other chlorophyll a/c-containing phytoplankton. Here we report the isolation and cloning of a plastocyanin gene from Thalassiosira oceanica (CCMP 1005). The gene encoded a Cu-containing protein that is known in other organisms to functionally replace the Fe-containing cyt c6. The inferred protein sequence had the highest identity with the green haptophyte, Emiliania huxleyi, and possessed many of the globular properties necessary for function and interaction with upstream and downstream partners. Eleven strains of oceanic and coastal diatoms were screened for the presence of the plastocyanin gene using degenerate primers: one other species was observed to contain the gene; T. oceanica (CCMP 1006). Phylogenetic analysis of the 5.8 rRNA gene of these species showed that both T. oceanica strains with plastocyanin were closely related to each other. The cloned sequence of T. oceanica (CCMP 1006) contained greater than 80% of the protein-coding region and shared 99% nucleotide identity and 100% conserved unique intronic region. The inferred protein sequence of this species had 100% identity with the inferred protein sequence of T. oceanica (CCMP 1005).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".