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Record W6986276683

Overexpression of cytosolic glutamine synthetase in rice improves utilization nitrogen use efficiency

2009· dissertation· en· W6986276683 on OpenAlexfundno aff

Bibliographic record

VenueThe Atrium (University of Guelph) · 2009
Typedissertation
Languageen
FieldAgricultural and Biological Sciences
TopicPlant nutrient uptake and metabolism
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsGlutamine synthetaseCytosolNitrogenNitrogen assimilationGlutamineShootEnzymeAmmoniaTransgene
DOInot available

Abstract

fetched live from OpenAlex

This thesis tests the hypothesis that overexpression of cytosolic glutamine synthetase (GS1) in rice improves utilization nitrogen use efficiency (UtE). GS1 is responsible for the primary assimilation of ammonia and a role in nitrogen remobilization is implicated from its vascular localization and enhanced expression during senescence. Three GS1 overexpressor lines were identified using activity assays and quantitative real-time polymerase chain reaction. Physiological analysis of the lines was conducted at varying nitrate conditions, growth stages and environments. Overall, overexpressor lines did not differ from azygous controls in shoot biomass or nitrogen accumulation. However, harvest index and UtE were significantly enhanced in some of the transgenic lines relative to their corresponding azygous controls. These characteristics were highly correlated with percent spikelets filled, spikelet number and yield. Thus, nitrogen partitioning in rice during grain filling can be altered by GS1 overexpression, resulting in improved UtE.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.215
Teacher spread0.193 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2009
Admission routes1
Has abstractyes

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