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Record W6986718933

Prosaposin: a protein with\n\t\t\t\t differential sorting and multiple functions

2020· article· en· W6986718933 on OpenAlexafffund

Bibliographic record

VenueLA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCellular transport and secretion
Canadian institutionsMcGill University
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsActivator (genetics)Endoplasmic reticulumGolgi apparatusExtracellularReceptorNervous systemSecretory pathwayGlycoproteinGalectin
DOInot available

Abstract

fetched live from OpenAlex

In eukaryotes the delivery of newly\n\t\t\t\t synthesized proteins to their final destination is\n\t\t\t\t dependent on a series of functionally distinct\n\t\t\t\t compartments, including the endoplasmic reticulum and\n\t\t\t\t the Golgi apparatus, which plays a role in posttranslational modification, sorting and distribution of\n\t\t\t\t proteins. Most cargo is sorted within, and exits from, the\n\t\t\t\t trans-Golgi network (TGN). Proteins delivered to\n\t\t\t\t lysosomes include hydrolytic enzymes and nonenzymic\n\t\t\t\t activator proteins. They are directed away from the cell\n\t\t\t\t surface by their binding to mannose-6-phosphate\n\t\t\t\t receptors (MPR). However, in I-cell disease, in which\n\t\t\t\t the MPR pathway is disrupted, the nonenzymic\n\t\t\t\t sphingolipid activator protein, prosaposin, continue to\n\t\t\t\t traffic to lysosomes. This observation led to discovery of\n\t\t\t\t a new lysosomal sorting receptor, sortilin. The targeting\n\t\t\t\t prosaposin to the lysosomes results from the interaction\n\t\t\t\t of its C-terminus with sortilin. Deletion of the Cterminus did not interfere with its secretion, but\n\t\t\t\t abolished its transport to the lysosomes. Mutational\n\t\t\t\t analysis revealed that the first half of the prosaposin Cterminus contains a motif required for its binding to\n\t\t\t\t sortilin and its transport to the lysosomes. Prosaposin\n\t\t\t\t can be also secreted to the extracellular space as\n\t\t\t\t oligomers. Extracellular prosaposin showed to exert a\n\t\t\t\t variety of responses in nervous tissues including the\n\t\t\t\t activation of G protein-coupled receptors and ERK\n\t\t\t\t phosphorylation. Lastly, prosaposin has been found to be\n\t\t\t\t expressed in other fluids of the body such as pancreaticjuice, bile, cerebrospinal fluid, milk and seminal fluid,\n\t\t\t\t indicating that prosaposin is not only a house keeping\n\t\t\t\t lysosomal protein but an essential factor in the\n\t\t\t\t development and maintenance of the nervous systems\n\t\t\t\t and other systems of the body.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.212
Teacher spread0.197 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2020
Admission routes2
Has abstractyes

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