Functional characterization of stomatin-like protein 2
Bibliographic record
Abstract
Stomatin-like protein (SLP)-2 is a widely expressed and highly conserved protein identified in a proteome analysis of detergent-insoluble, glycolipid-enriched microdomains of human T cells activated through the antigen receptor.SLP-2 partitions mostly in mitochondria but a low abundance pool is present at the plasma membrane.SLP-2 is upregulated upon T cell activation, and its deletion in T cell-specific SLP-2 knockout mice results in a post-transcriptional defect in IL-2 production and decreased T cell responses.Mechanistically, SLP-2 binds the mitochondrial phospholipid cardiolipin and interacts with prohibitin (PHB) proteins, correlating with impaired cardiolipin compartmentalization and respiratory function in SLP-2-deficient T cells.In our current model of SLP-2 function, SLP-2 recruits PHBs to cardiolipin to form cardiolipinenriched microdomains in the mitochondrial inner membrane.We hypothesize that these specialized microdomains may provide an environment necessary for the optimal assembly and function of multichain complexes, including respiratory supercomplexes and translation machinery.Respiratory chain complexes naturally associate to form supercomplexes for optimal electron transfer and function, and cardiolipin is known to be important in this process.We found that in the absence of SLP-2, T cells exhibited defective assembly of complex I-III 2 and I-III 2 -IV 1-3 supercomplexes but not of individual respiratory complexes.This correlated with increased respiration uncoupled from ATP production, a greater reliance on glycolysis, and significantly delayed T cell proliferation when glycolysis was inhibited, together demonstrating the importance of SLP-2 in mitochondrial respiratory function.Mitochondrial translation occurs in close proximity to the mitochondrial inner membrane and is essential for generating core polypeptides of respiratory chain complexes.Given the Table of Contents
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".