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Record W6991453931

Functional Analysis of Neutrophil Cytosolic Factor 4 (NCF4) in the pathogenesis of Pediatric Inflammatory Bowel Disease

2018· dissertation· en· W6991453931 on OpenAlexaff

Bibliographic record

VenueTSpace (University of Toronto) · 2018
Typedissertation
Languageen
FieldImmunology and Microbiology
TopicNeutrophil, Myeloperoxidase and Oxidative Mechanisms
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsNADPH oxidaseChronic granulomatous diseaseSuperoxidePathogenesisInflammatory bowel diseaseOxidase testIntracellularRespiratory burstApocynin
DOInot available

Abstract

fetched live from OpenAlex

Genetic variants in NADPH oxidase are known to increase susceptibility of Very Early Onset Inflammatory Bowel Disease (VEOIBD). NADPH oxidase generates superoxide anions for bactericidal activity in phagocytes. Defects in NADPH oxidase lead to Crohn’s disease (CD) and Chronic Granulomatous Disease (CGD) like phenotypes, characterized by defective respiratory burst and inefficient pathogen clearance. This study evaluates the functional consequences of an autosomal recessive nonsense variant in NCF4 in the onset of pediatric IBD. It truncates p40phox (p40phox (W239X)), a subunit of the NADPH oxidase. It is reported that p40phox (W239X) loses its interaction with its known binding partner p67phox in NADPH oxidase and mislocalizes to early endosomes; instead of the cytoplasm. Impaired intracellular oxidative burst and reduced bactericidal activity were observed in macrophages stably expressing p40phox (W239X). It is proposed that p40phox (W239X) does not interact with NADPH oxidase causing its functional impairment and prompting the onset of pediatric IBD.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.226
Teacher spread0.212 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2018
Admission routes1
Has abstractyes

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