How Fusarium graminearum affects intermediate wheatgrass and its mycobiome
Bibliographic record
Abstract
An emerging crop, intermediate wheatgrass (IWG) is resistant to the natural occurrence of most important cereal diseases and is currently under domestication to improve its yield. An IWG population consisting of two parental lines and 154 individual F1 progenies were inoculated with F. graminearum spores in a field setting to check their performance against Fusarium head blight (FHB) over two consecutive years. The higher humidity in 2022 lead to higher FHB severity and incidence, and higher DON (deoxynivalenol) mycotoxin accumulation than in 2021. DON was mostly restricted to the chaff in 2021, whereas in 2022, DON was found in the grain as well. Considering all the FHB aspects checked in this experiment, four IWG progeny individuals (plants: P-17, P-43, P-107, and P-139) were found to consistently perform better than the parental lines and the other progeny individuals during both years. The second part of this thesis analyzed the influence of F. graminearum on the mycobiome of IWG spikelet and flag leaf samples from two different locations, Winnipeg and Carman, MB. A total 372 IWG samples (half of which were inoculated with F. graminearum [Winnipeg] and the other half, not [Carman]) were used for fungal community profiling via ITS amplicon sequencing using PacBio technology. There were significant differences in the fungal community among the IWG spike samples from Winnipeg and Carman. As expected, F. graminearum dominated IWG spike mycobiomes at the inoculated Winnipeg site, whereas in Carman, Alternaria dominated. Also, the presence of F. graminearum seemed to influence the broader spike mycobiome and the presence of beneficial fungi was less where F. graminearum dominated. The mycobiome data also revealed that the pathogenic fungi Alternaria, Nigrospora, Septoriella, Stemphylium, Claviceps, Bipolaris, and Drechslera can become potential threats to IWG in the future. The last part of this thesis sheds light on the interaction between F. graminearum and another agriculturally important fungus, Claviceps purpurea. In planta interactions showed that C. purpurea can negatively affect infection by F. graminearum only if C. purpurea was introduced first into the plant site, but further investigation of this is necessary for confirmation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".