Isolation and characterization of mutants affecting carbon catabolism in Sinorhizobium meliloti
Bibliographic record
Abstract
Sinorhizobium meliloti is a bacterium capable of participating in a symbiotic relation- ship with plants in which it forms N2 fixing nodules.The ability to use specific carbon sources has been shotnm to provide S. melíIoti and other rhizobia with competitive ad- vantages in the ability to effectively nodulate host plants.The sugars rhamnose and ara- binose were hypothesized to play a role in the abitity to compete for nodule occupancy in S. melilori.To test these hypotheses, the S. meliloti wild type, RmI02l, was subjected to transposon mutagenesis experiments and the resulting mutants were screened for the inability to use rhamnose or arabinose as sole carbon sources.Mutations affecting rhamnose and arabinose catabolism were isolated and subsequent characterization re- sulted in the identification of a rhamnose catabolism operon, an arabinose catabolism operon and a triose phosphate isomerase encoding gene.A-lthough mutants unable to utilize arabinose were not less competitive than wild type, the ability to utilize rhamnose was found to play a role in competition for nodule occupancy.Furthermore, the roles of two triose phosphate isomerase genes were characterized with respect to symbiosis and the free-living state.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".