Tripartite «trans»-splicing of the L1.LtrB group ll intron
Bibliographic record
Abstract
Group II introns are ribozymes that self-splice from pre-mRNA transcripts, concurrently ligating their flanking exons. From an evolutionary perspective, they are considered to be the progenitors of eukaryotic nuclear introns and their splicing machinery, the spliceosome. It is hypothesized that the fragmentation of an ancestral group II intron gave rise to the five small nuclear RNAs (snRNAs - U1, U2, U4, U5, U6) that constitute the catalytic core of the spliceosome. One factor lending support to this hypothesis is the ability of fragmented organellar group II introns to splice in trans. We subjected the Ll.LtrB group II intron from the Gram-positive bacterium Lactococcus lactis to an EZ-Tn5-based fragmentation screen and selected for proficient tripartite trans-splicers through a trans-splicing/conjugation assay. By demonstrating the first tripartite trans-splicing Ll.LtrB variants in vivo, we provide further experimental support for the evolutionary link between group II introns and the snRNAs of the eukaryotic spliceosome.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.004 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".