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Record W6997248100

The Use of Magnetotactic Bacteria to Remove Phosphorus from Eutrophic Conditions

2018· dissertation· en· W6997248100 on OpenAlexaboutno aff

Bibliographic record

VenueThe Knowledge Bank (The Ohio State University) · 2018
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGeomagnetism and Paleomagnetism Studies
Canadian institutionsnot available
Fundersnot available
KeywordsEutrophicationPhosphorusMicroorganismNutrientWater pollutionNitrogen
DOInot available

Abstract

fetched live from OpenAlex

Eutrophication or excess nutrients in rivers and lakes is a problem in the Midwest commonly caused by high levels of phosphorus in runoff from agricultural land. Magnetotactic bacteria (MTB), a magnetite-containing microorganism found in aquatic ecosystems, may contain intracellular inclusions of phosphorus. This study will test how effective MTB is at removing high concentrations of phosphorus from eutrophic conditions. The hypothesis for this project is that MTB will have some capability to remove phosphorus from their water environments, offering a microbiological solution in places where eutrophication occurs. Methods include growing the type strain of MTB, Magnetospirillum magneticum, AMB-1, in media spiked with concentrations of phosphorus at 0.01 mg/L, 0.025 mg/L, and 0.06 mg/L. These concentrations were selected to mimic, respectively, Canada's target level of phosphorus for Lake Erie, the US Environmental Protection Agency's target level of phosphorus in lakes, and the peak phosphorus levels found in the western Lake Erie basin in 2010. A colorimetric analysis was used to measure phosphorus in solution at different time points. A centrifuge was used to separate the cells from the media. Results indicate that when phosphorus-containing media is inoculated, concentrations of phosphorus decrease in the media after two days. Samples with higher concentrations of phosphorus experience more rapid decreases in solution phase phosphorus. Phosphorus was recovered from the cell pellet, indicating phosphorus was removed and stored in AMB-1 cells. Results indicate this technology may hold some promise for limiting eutrophic conditions such as those that occur in northwest Ohio and Lake Erie.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.603
Threshold uncertainty score0.975

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.225
Teacher spread0.210 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2018
Admission routes1
Has abstractyes

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