The Characterization of Taz Protein-Protein Interactions in Myogenic Cells
Bibliographic record
Abstract
The Hippo signaling pathway is known to regulate cell proliferation, differentiation, and apoptosis. Recent studies identified the Hippo signaling target protein TAZ as a repressor of myogenic differentiation. Since TAZ lacks inherent DNA binding ability, its activity is dependent on its protein interactions. Using GFP-nanoTrap affinity purification and LC-MS/MS, we documented TAZ protein interactions in muscle cells, including the pro-myogenic methyltransferase CARM1. Investigation of the TAZ and CARM1 interaction revealed that CARM1 represses TAZ transcriptional activation, promoting TAZ Ser89 phosphorylation and cytoplasmic sequestration. The TAZ/CARM1 interaction was further investigated in embryonic rhabdomyosarcoma (RD) cells, where Hippo signaling increased compared to control C2C12 myoblasts. Further, mass spectrometry results suggest that TAZ is a substrate of CARM1 methylation, with methylation at Arg 77 enhancing TAZ Ser89 phosphorylation. These findings highlight the potential role of CARM1 in regulating TAZ function in myogenic cells.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".