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Record W6999258632

Chromatin condensation and fragmentation caused by CUG-initiated FGF-2 in cardiomyocytes

2000· other· en· W6999258632 on OpenAlexvenueno aff

Bibliographic record

VenueLibrary and Archives Canada (Government of Canada) · 2000
Typeother
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicFibroblast Growth Factor Research
Canadian institutionsnot available
Fundersnot available
KeywordsChromatinProphaseFragmentation (computing)DNAPhenotypeFibroblastGene
DOInot available

Abstract

fetched live from OpenAlex

Fibroblast growth factor-2 (FGF-2) is a multifunctional factor which is found in 21-25 kDa high molecular weight (HMW), or 16-18 kDa low molecular weight (LMW) forms, resulting from translation initiation from unconventional CUG- or conventional AUG- start sites, respectively. Previous studies have demonstrated that rat HMW- but not LMW-FGF-2, introduced into cardiac myocytes by gene transfer, caused a distinct nuclear phenotype, characterized by condensed chromatin and formation of several DNA 'clumps' inside the nucleus. In the present study we investigated first whether human HMW FGF-2, sharing 82% homology with its rat counterpart at the CUG-initiated extension, was also capable of eliciting the same phenotype as rat HMW FGF-2. Secondly, we investigated whether the mechanism of chromatin condensation and separation caused by HMW FGF-2 engaged an apoptosis-like, mechanism. Finally, we examined whether the HMW FGF-2 induced nuclear phenotype resulted from a mitosis-like mechanism. (Abstract shortened by UMI.)

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.527
Threshold uncertainty score0.978

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.004
GPT teacher head0.170
Teacher spread0.166 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2000
Admission routes1
Has abstractyes

Explore more

Same venueLibrary and Archives Canada (Government of Canada)Same topicFibroblast Growth Factor ResearchFrench-language works237,207