A novel role of the vaccinia virus mRNA capping enzyme in determining virus host range
Bibliographic record
Abstract
E3 family proteins are a poxvirus defense gene that have a significant role in host range determination. Their biological function has largely been attributed to the ability of the protein to bind double-stranded RNA. Increasing evidence indicates this mechanism is more complex. For example, the ortholog, SPPV034, is unable to rescue replication of a recombinant virus even though it retains the ability to bind dsRNA. The Vaccinia virus capping enzyme (the D1-D12 heterodimer) is a cofactor for E3 protein in determining host range. A D1-G801S mutation rescued replication of the SPPV034 recombinant virus and inhibited activation of host responses. A D12-S131N partially rescue replication, but could not fully antagonize host responses. These results indicate that E3 proteins operate on a threshold mechanism determined by intrinsic properties. The D1-D12 heterodimer mutations altered the quantity of dsRNA produced during infections so that the upper limit of the SPPV034 protein was not reached.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".