Production of mutants exhibiting cold tolerance in spring canola (Brassica napus)
Bibliographic record
Abstract
A major factor affecting spring canola ('Brassica napus') production in Canada is cold temperatures and killing frosts during seed development or during seed maturation. The objective of this study is to produce mutations ' in vitro' that have altered biochemical pathways that increase cold tolerance. To enhance the understanding of cold stress in canola in the cold-induced oxidative stress pathway, mutants with elevated levels of key defense signaling molecules salicylic acid (SA) and jasmonic acid (JA) were produced using UV light. In addition, mutants that overproduce proline were produced, which is known to protect plant tissues in the cold-induced osmotic stress pathway. There were nineteen out of the seventy-four mutants tested in the final controlled environment indoor freeze test identified with significant cold tolerance compared to their donor parents. Two proline selected mutants, 997-48 and 997-71 both had greater survival percentages in winter field trials over multiple years as compared to parental and winter checks. Development of increased frost tolerant cultivars should allow for spring canola to be produced in western Canada without compromising seed quality.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".