The role of TUDOR in «Drosophila» polar granule assembly and germ cell formation
Bibliographic record
Abstract
Germ cells are a totipotent stem cell line where meiosis occurs; without germ cell formation sexual and types of asexual reproduction cannot occur. Drosophila germ cell formation depends on a specialized cytoplasm which is at the posterior of early embryos. The germ plasm in Drosophila contains electron dense structures called polar granules. Similar electron dense structures are found in the germ line of many metazoan species. This thesis examines the polar granule component, TUDOR and its molecular function in Drosophila germ cell formation and polar granule assembly. I created a null allele of tud and found that without TUDOR, embryos are incapable of forming germ cells, while some embryos had normally patterned abdomens. As TUDOR was dispensable for somatic functions but central to germ cell formation, I isolated TUDOR containing complexes from embryos. I then compared the proteins found in my TUDOR complexes to those in VASA complexes to determine which TUDOR complex components are polar granule constituents. I examined five proteins in the TUDOR and VASA complexes, TER94, AUBERGINE, Me31B, eIF4A and Pyruvate Kinase. Reduction of VASA or TUDOR from the early embryo along with TER94, Me31B, eIF4A or AUBERGINE results in a decrease in germ cell formation. As well, TER94, Me31B, eIF4A and AUBERGINE all localize to polar granules. Three of the polar granule components we isolated, Me31B, eIF4A and AUBERGINE, are proteins typical of P bodies, sites of mRNA metabolism, suggesting that polar granules might be related to P bodies specific. Interestingly, immuno-electron micrographs of TER94, an endoplasmic reticulum (ER) component, showed that polar granules can associate with ER. We also show Pyruvate Kinase, a glycolytic protein
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".