The Role of Choline in Gestational Diets in the Programming of Food Intake Regulation and Metabolism in Wistar Rat Offspring
Bibliographic record
Abstract
High (5-fold) folic acid (FA) diets during pregnancy result in obesity in rat offspring, associated with altered DNA-methylation of hypothalamic food-intake neurons. Like FA, the methyl-donor choline modulates fetal brain development, but its long-term programming effects on metabolic phenotype has not been reported. The hypothesis of this research is that choline in the gestational diet, like FA, is a contributor to the in utero programming of hypothalamic food intake regulation, and the later-life metabolic phenotype of male Wistar rat offspring. In study 1, Pregnant Wistar rats were fed an AIN-93G diet with recommended choline (RC, 1 g/kg diet), low choline (LC, 0.5-fold), or high choline (HC, 2.5-fold). Male offspring were weaned to a normal (16%) fat diet for 17 weeks. In study 2, the interaction between the choline content of the gestational diet (RC vs HC) and fat content of the post-weaning diet (normal (16%) vs high (45%)) on metabolic phenotype of male Wistar rats was assessed. In study 3, pregnant Wistar rats were fed an AIN-93G diet with recommended choline and FA (1X, RCRF, control), or high (5X) FA with choline at 0.5X-(LCHF), 1X-(RCHF), or 2.5X-(HCHF). Male offspring were weaned to the control diet for 20 weeks. In study 1, choline content in the gestational diet (below or above recommended intakes) programmed changes in food intake, body weight, energy expenditure and hypothalamic energy-regulatory neuropeptides. In study 2, choline additions reduced long-term body weight-gain and improved the later-life metabolic phenotype of offspring fed a high fat, but not a normal fat, post-weaning diet. In study 3, high gestational choline reduced high FA induced increases in long-term food intake and body weight and modified expression of regulatory genes in the hypothalamus. In studies 1 and 3, both early and later-life brain and plasma 1-carbon metabolites were affected by the choline content in gestational diets. In conclusion, maternal choline contributes to the in utero programming of hypothalamic food intake regulation and the metabolic phenotype of male rat offspring, and its effects are dependent on the composition of the gestational and post-weaning diets.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".