Tissues-on-a-string: Analyzing transport within tissues via perfusable glass-sheathed hydrogel microtubes
Bibliographic record
Abstract
Measuring transport of soluble molecules such as nutrients, waste, and therapeutics within tissues is essential to understand the impact of culture dimensionality on tissue function, and to realize improved 3D cell culture discovery platforms.Studying these properties on conventional 3D cell cultures poses great challenges as the delivering or sampling access within these complex 3D tissue structures is either challenging to precisely position within the desired region or simply destructive.In this thesis, we demonstrate proof-of-concept for a simple technique to both deliver and sample soluble factors from a point source within spheroids, using a highly perfusable hollow-core hydrogel microtube and an integrated glass capillary sheathing system, that successfully limits transport to a well-defined region inside the tissue.We successfully demonstrated its applicability by analyzing the transport properties of a model placental trophoblast choriocarcinoma, cultured as a 3D spheroid.Through comparison with finite element modeling of soluble diffusion in our platform, we found that the diffusivity within placental spheroid is heterogeneous as it shows higher diffusivity towards its core and gradually decreases the edge.This platform provides a simple design that could be used to rapidly study diffusion of soluble within a 3D tissue.the heartful energy you brought to the lab.To Karthick, thank you for all the projects we shared and help I got from you.To James, thank you for starting master's with me and sharing common interests even though we are so different (I still can't forgive you for hating poutine).To Ben and Nick W. (no.3), thank you for being fun officemates and losing money in stocks with me and specifically to Ben for saving me countless times in process control and to Nick for trusting me in my meme stocks.To Scott, thank you for the amazing food you made at the chalet.Outside of the lab, I would like to thank all my badminton friends and teammates.The cheerful energy I get from them during competitions, trainings and social events allowed me stay healthy both physically and mentally.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".