The architecture of the ERI complex involved in initiating endogenous RNA interference in «C.elegans»
Bibliographic record
Abstract
RNA interference (RNAi) is a gene silencing mechanism occurring in a wide variety of organisms. Different RNAi pathways exist in C. elegans for the regulation of various endogenous processes. The ERI-mediated RNAi pathway involves the ERI proteins and C. elegans Dicer, DCR-1, suggested to be involved in initiating endogenous RNAi. In this study, it was observed that many of the ERI proteins co-fractionate with DCR-1 in a complex of ~885 kDa in size. It was shown that the RdRP RRF-3 and the Tudor domain protein ERI-5 require each other in order to associate with DCR-1, but independently of ERI proteins ERI-1 and ERI-3. Furthermore, it was also observed that the helicase DRH-3 requires RRF-3 and ERI-5 in order to associate to DCR-1. The data suggested that at least two distinct binding sites for ERI proteins are found on DCR-1. Preliminary GST pulldown data confirmed distinct binding regions on DCR-1 for ERI-5 and ERI-3.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".