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Record W7009494119

The Effects of Dexamethasone on Estrogen Cell Signaling Pathways in the mHippoE-14 Cell Line & Female Rats

2023· dissertation· en· W7009494119 on OpenAlexaff

Bibliographic record

VenueThe Atrium (University of Guelph) · 2023
Typedissertation
Languageen
FieldMedicine
TopicMenopause: Health Impacts and Treatments
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsMAPK/ERK pathwayDownregulation and upregulationPhosphorylationGlucocorticoidCell cultureEstrogenSignal transductionDexamethasone
DOInot available

Abstract

fetched live from OpenAlex

Previous research in our lab has demonstrated alterations in molecular markers of hippocampal structure and function in response to stress, including DUSP6 expression, as well as ERK and JNK phosphorylation (Nicholson 2019, Craig 2021, Pacosz 2021). To further characterize JNK phosphorylation, ERK phosphorylation, and DUSP6 expression we ran two experiments, one in the mHippoE-14 cell line, and one in cycling female rats in metestrus. A significant downregulation of p54 JNK phosphorylation was found in the mHippoE-14 cell line in response to the combination of 17β-estradiol (E2) pretreatment and dexamethasone treatment, although no significant effects were found in the cycling female rats. Altogether, the data from this thesis suggests that circulating levels of E2 in metestrus are not sufficient to reverse or attenuate glucocorticoid induced effects on DUSP6 expression, ERK phosphorylation, and JNK phosphorylation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.037
GPT teacher head0.278
Teacher spread0.241 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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