A forward genetic screen for enhancers of «ksr-1» lethality
Bibliographic record
Abstract
Kinase suppressor of Ras (KSR) is a scaffold protein that is required to localize components of the Ras/ERK pathway to the plasma membrane. In C. elegans, two genes, ksr-1 and ksr-2, encode KSR proteins which are redundantly required for development. Less than 1% of ksr-1(-) animals die in L1 larval stage in a distinct rod-like manner, but additional loss of ksr-2 or other positive regulators of the pathway enhance the rod-like lethal phenotype. To identify addition genes that might function with KSR, we performed a forward genetic screen to isolate genes that enhance ksr-1 rod-like lethality. From the screen, six enhancers were isolated, three of which are alleles of an E2 ubiquitin conjugating enzyme, ubc-25. Additionally, three alleles of an unknown gene on chromosome X were isolated but are not enhancers of ksr-1 rod-like lethality. These new enhancers as well as the mutations on chromosome X might define new regulators of Ras/ERK signaling.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".