Identifying the Ecological Interactions of Invasive Lymantria Dispar Dispar and Agrilus Planipennis Using Metabarcoding Techniques
Bibliographic record
Abstract
The objectives of this thesis were to develop a standardized metabarcoding protocol to identify ecological interactions between insects and other taxa, as well as determine the impact of ecological correlates on the detection of these interactions. In Chapter 2, I evaluate target loci for the identification of Lymantria dispar dispar (spongy moth) and Agrilus planipennis (emerald ash borer) ecological interactions and develop a standardized protocol for the analysis of these interactions. I demonstrate that my metabarcoding protocol can be used to identify a wide diversity of interactions, including those with animals, plants, bacteria, and fungi. In Chapter 3, I assess how ecological factors (e.g., life stage, collection location, etc.) influence the presence or absence of ecological interactions in emerald ash borers using random forest models. I found that interactions were more likely to be detected in pupal specimens and in samples collected earlier in the year.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".