Molecular and biological characterization of turkey coronavirus
Bibliographic record
Abstract
Turkey Coronavirus (TCoV) is an important enteric pathogen affecting commercial turkeys. The complete genome sequence of a TCoV isolate from Ontario (27,657 bases) was determined (assigned as the reference sequence for TCoV GenBank NC_010800). The genome organization of this virus was determined to be 5'-UTR-Pol-S-3a-3b-E-M-ORF-X-5a-5b-N-UTR-3'. Based on these findings, TCoV was assigned to group III coronaviruses. Two structural proteins (nucleocapsid and truncated spike glycoprotein) were expressed in 'Escherichia coli' and used to develop diagnostic ELISAs for TCoV antibodies. Serum samples from Ontario turkey farms tested using both recombinant protein ELISAs revealed high seroprevalence of TCoV. To study TCoV infection, an experimental trial was conducted in which 2-day-old turkey poults (20 birds) were inoculated orally with the Ontario TCoV isolate. Birds developed clinical signs of depression, anorexia and obvious diarrhea. Body weight gain of infected birds was significantly decreased in comparison to the body weight gain of uninfected controls. Virus shedding was monitored using TCoV-specific RT-PCR. TCoV shedding in the feces of infected birds started at 24 hours post-infection, continued for a period of up to 17 days, and ceased completely by 21 days post-infection (dpi). The recombinant TCoV antigen-based antibody ELISAs detected the seroconversion at 10-14 dpi. TCoV-specific antibodies could be detected in the serum of all infected poults by 3 weeks post infection. Poults recovered from an infection acquired at 2 days of age were refractory to re-infection by oral/intramuscular challenge at 21 days of age indicating that protective immunity was elicited relatively quickly.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".