Regulation of phosphorylation of protein kinace C in neurons
Bibliographic record
Abstract
In Aplysia, persistent increases in synaptic strength are mediated by persistent activation of protein kinase C. In particular, the novel PKC Apl II is persistently active between 90 minutes and 2 hours after training. Although unknown, the most likely mechanism of persistent activation is phosphorylation. We characterized phosphorylation of PKC Apl II at a conserved hydrophobic site, serine 729, with a phosphospecific antibody. Phosphorylation of S729 increased and persisted 2 hours after serotonin treatment, but did not change after phorbol ester activation. Furthermore, a serine 729 to alanine mutation reduced the activity and stability of ApI II. We studied upstream regulation of S729 phosphorylation, and showed that it is affected by both PDK-1 and atypical PKC Apl III in SF9 cells but not by FRAP or PtdIns3K in neurons. Finally we characterized atypical PKC Apl III using immunoblotting, and used kinase assays to identify isoform specific inhibitors for future studies.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.009 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".