Using «in vivo» and «in vitro» tools to investigate the role of adhesion in cellular morphogenesis
Bibliographic record
Abstract
N-cadherin is a prominent cell adhesion molecule involved in developmental morphogenesis and synapse formation. We transgenically overexpressed N-cadherin in a spatially and temporally specific manner using the Cre-loxP system. We overexpressed N-cadherin following the peak developmental period for synaptogenesis using the CamKIIa promoter to explore the effects of N-cadherin upregulation on synapse formation. N-cadherin, in concert with other proteins, plays an important role of adhesion in synapse formation and maintenance. Overexpressing N-cadherin in the hippocampus led to an upregulation of specific components of the synaptic complex; β-catenin, PSD-95, GluN2B and synaptophysin were elevated at the protein level whereas the GABAAR, a marker of inhibitory synapses, was unchanged. Dendritic spine length was reduced in overexpressors while spine diameter and density were indistinguishable from wild-type. These results demonstrate that N-cadherin plays an important role in the regulation of synaptic properties.During development, cell morphogenesis occurs in response to cues that can be permissive or inhibitory. Using microcontact printing we created complex microenvironmental conditions to mimic those observed in vivo to examine their impact on cell growth and differentiation. In this study we used a known growth permissive substrate, polylysine (PLL) and an inhibitory substrate, myelin to examine growth responses of neuronal as well as non-neuronal cells to complex environments. We showed that micropatterning of PLL can be used to direct the adherence and axonal outgrowth of hippocampal and cerebellar neurons as well as other cells such as Oli-neu oligodendrocyte progenitor cell lines and fibroblast-like COS7 cells in culture. By printing myelin in stripes, we were able to show that myelin could impair process outgrowth of Oli-neu cells and that this effect was reversible by using Y-27632 a ROCK inhibitor. Use of micro-engineering techniques provides tools to study the mechanisms underlying diverse cell behaviors on patterned permissive and non-permissive substrates.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.003 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".