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Record W7016093589

In vitro characterization of the role of chalcone isomerase-like (CHIL) in flavanone biosynthesis

2023· dissertation· en· W7016093589 on OpenAlexfundno aff

Bibliographic record

VenueeScholarship@McGill (McGill) · 2023
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant Gene Expression Analysis
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of CanadaMcGill University
KeywordsFlavanoneBiosynthesisIn vitroChalconeEnzyme
DOInot available

Abstract

fetched live from OpenAlex

Analysis of variance ATP Adenosine triphosphate BiFC Bimolecular fluorescence complementation BNY Bisnoryangonin bp Nucleic acid base pairs BSA Bovine serum albumin C4H Cinnamate 4-hydroxylase CHI Chalcone isomerase CHIL Chalcone isomerase-like CHR Chalcone reductase CHS Chalcone synthase CoA Coenzyme-A COR Codeinone reductase CTAL p-Coumaroyltriacetic acid lactone CTAS p-Coumaroyltriacetic acid synthase DDC Duplication, degeneration, and complementation DIMBOA 2,4-Dihydroxy-7-methoxy-1,4-benzoaxazin-3-one DMX Desmethylxanthohumol DNA Deoxyribonucleic acid DSF Differential scanning fluorimetry ECL Enhanced chemiluminescence EFP Enhancer of flavonoid production ER Endoplasmic reticulum FAP Fatty acid-binding protein FNS Flavone synthase HCl Hydrochloric acid HCT Hydroxycinnamoyl transferase hER Human estrogen receptor His Histidine HRP Horseradish peroxidase HSD Honestly significant difference IFS Isoflavone synthase IPTG Isopropyl-β-D-1-thiogalactopryranoside * Phytozome v13.0 (https://phytozome-next.jgi.doe.gov/)gene identifiers.† Expected molecular weight (kDa), including N-terminal tags.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.216
Teacher spread0.210 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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