Alendronate affects calcium dynamics in cardiomyocytes
Bibliographic record
Abstract
Alendronate (ALN) is effective in the treatment of osteoporosis. However, ALN has been recently associated with an increased risk of serious atrial fibrillation. We investigated the effects of ALN on cytosolic free calcium concentration ([Ca2+]i) in cardiomyocytes. HL-1 atrial cardiomyocytes were loaded with fura-2 and examined using microspectrofluorimetry. ALN (10-8, 10-7, 10-6 M) induced transitory high frequency oscillations of [Ca2+]i with greater frequency for 10-8 M ALN (61 ± 10 mHz) compared to 10-6 ALN (42 ± 4 mHz). In cells treated with 10-6 M ALN responses to subsequent application of caffeine were delayed, and exhibited a decrease in the rate and amplitude of [Ca2+]i increase. Long term (48 h) exposure to 10-8 M Alendronate resulted in delay of caffeine-induced Ca2+ transients and decreased rate of [Ca2+]i increase, followed by oscillations in [Ca2+]i of 54 ± 8 mHz versus those observed at higher concentrations of Alendronate (35 ± 5 mHz). Changes in calcium dynamics were accompanied by significant changes in the expression of sarcoendoplasmic reticulum ATPase (SERCA2a), calsequestrin and calreticulin.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".