Assessing the Leaf Blade Nutrient Status of Pinot Noir Using Hyperspectral Reflectance and Machine Learning Models

Monitoring grape nutrient status, from flowering to veraison, is important for viticulturists when implementing vineyard management strategies, in order to produce quality wines.However, traditional methods for measuring nutrient elements incur high labour costs.The aim of this study is to explore the potential of predicting grapevine leaf blade nutrient concentration based on hyperspectral data.Leaf blades were collected at two Pinot Noir commercial vineyards at Martinborough, New Zealand.The leaf blade spectral data were obtained with a handheld spectroradiometer, to evaluate surface reflectance and derivative spectra in the spectrum range between 400 and 2400 nm.Afterwards, leaf blades nutrient concentrations (N, P, K, Ca, and Mg) were measured, and their relationships with the hyperspectral data were modelled by machine learning models; partial least squares regression (PLSR), random forest regression (RFR), and support vector regression (SVR) were used.Pearson correlation and recursive feature elimination, based on cross-validation, were used as feature selection methods for RFR and SVR, to improve the model's performance.The variable importance score of PLSR, and permutation variable importance of RFR and SVR, were used to determine the most sensitive wavelengths, or spectral regions related to each biochemical variable.The results showed that the best predictive performance for leaf blade N concentration was based on PLSR to raw reflectance data (R 2 = 0.66; RMSE = 0.15%).The combination of support vector regression with the Pearson correlation selected method and second derivative reflectance provided a high accuracy for K and Ca modelling (R 2 = 0.7; RMSE = 0.06%; R 2 = 0.62; RMSE = 0.11%, respectively).However, the modelling performance for P and Mg, by different feature groups and variable selection methods, was poor (R 2 = 0.15; RMSE = 0.02%; R 2 = 0.43; RMSE = 0.43%, respectively).Thus, a larger dataset is needed for improving the prediction of P and Mg.The results indicated that for Pinot Noir leaf blades, raw reflectance data had potential for the prediction of N concentration, while the second-derivative spectra were more suitable to predict K and Ca.This study led to the provision of rapid and non-destructive measurements of grapevine leaf nutrient status.

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