Development of field and microplate assays for evaluating DMI-fungicide resistance in Clarireedia jacksonii and other poaceous fungi
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Two assays for assessing Clarireedia jacksonii resistance to DMI fungicides and a propiconazole-tolerant microbiome in the Agrostis stolonifera phyllosphere were developed. The first assay allowed researchers and end users to identify resistant isolates from field samples using a selective medium (PDA amended with 0.5 µg/ml propiconazole, antibiotics, and tartaric acid). This medium was 96% successful in identifying resistant isolates from over 2000 inoculated leaf blades, and 92% successful in over 100 samples assessed by end users from 20 intensively managed turfgrass sites. The second assay used light absorbance to assess fungal growth in 96-well microplates with fungicide-amended media. The EC50 values of 32 isolates from the microplate assay were significantly correlated to those a traditional amended agar assay when both were log10 transformed (R = 0.75, p < 0.001). From the selective microbiome study, 19 propiconazole-tolerant fungi were isolated, five of which have not been previously associated with turfgrass phyllospheres.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it