Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui
Why this work is in the frame
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Bibliographic record
Abstract
Glutathione transferases (GSTs) obtained from University of Waterloo, Canada, were purified from Pseudomonas sp. UW4, by glutathione-affinity chromatography, identified through bioinformatic analysis and their substrate specificities were investigated. SDS-polyacrylamide gel electrophoresis revealed that the GST purified using Sulfobromophthalein-glutahione (BSP) affinity column resolved into a single band with low molecular weight (MW) of 17 kDa. Isoselectrofocusing showed it exists in single band with pI value of 6.1. Purified GST was reactive towards ethacrynic acid, 1-chloro-2,4-dinitrobenzene, cumene hydroxide and hydrogen peroxide, but no detectable activity with trans-2-octenal, hepta-2,4-dienal and trans-4-phenyl-3-butene-2-one. This demonstrated that putative GST possessed peroxidase activity but is not involved in lipid peroxidation. The purified GST suggested to be similar to PputUW4_00801 (putative glutathione S-transferase) of Pseudomonas sp. UW4.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it