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Record W7024396789

Role of miRNAs in Translational Control of Human Apolipoprotein B-100 mRNA

2013· dissertation· en· W7024396789 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueLibrary and Archives Canada (Government of Canada) · 2013
Typedissertation
Languageen
FieldComputer Science
TopicMultimodal Machine Learning Applications
Canadian institutionsnot available
Fundersnot available
KeywordsApolipoprotein BMessenger RNATransfectionmicroRNATranslational efficiencyTranslation (biology)Three prime untranslated regionTranslational regulation
DOInot available

Abstract

fetched live from OpenAlex

Apolipoprotein B (apoB) is a key structural and functional protein of lipoproteins\nand is synthesized constitutively in the liver. This study investigated the role of\nmicroRNAs (miRNAs) in translational control of apolipoprotein B (apoB) mRNA and\nprotein synthesis. Using bioinformatic analysis, I identified two specific miRNAs\nnamely, miR-544 and miR-1202 with potential to interact with 3’ and 5’ UTR of apoB,\nrespectively. Using HepG2 cells as the model system, the effects of transfection of\nexogenous miRNAs and inhibition of endogenous miRNAs were assessed on the\nexpression of apoB mRNA and protein synthesis, as well as apoB mRNA traffic into\ncytoplasmic P-bodies. miR-544 induced a significant reduction in apoB mRNA\nexpression and protein synthesis while increasing the co-localization of apoB mRNA into\nP-bodies. In contrast, transfection of miR-1202 increased apoB mRNA expression and\nprotein synthesis. In summary, these data demonstrate that specific miRNAs are involved\nin translational control of apoB mRNA.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.748
Threshold uncertainty score0.947

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.003
GPT teacher head0.173
Teacher spread0.170 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it