Afficheurs microfluidiques pour la caractérisation de la dynamique de la voie de signalisation cellulaire Notch
Bibliographic record
Abstract
RÉSUMÉ: La signalisation cellulaire est le processus biologique fondamental par lequel les cellules communiquent entre elles et avec leur environnement par l’entremise de différents signaux. La réponse cellulaire diffère selon la nature et l’intensité du signal, mais il est désormais connu que son évolution dans le temps a également un effet important. Notamment, la voie de signalisation Notch, importante dans le développement et le maintien de l’homéostasie, peut mener à des réponses cellulaires différentes selon sa dynamique d’activation. Ainsi, l’étude de la dynamique de signalisation cellulaire nécessite une résolution temporelle précise de livraison d’un signal aux cellules. Or, les techniques traditionnelles en biologie cellulaire axées sur le pipetage manuel des réactifs ne permettent pas d’atteindre cette résolution. Le laboratoire de Pr Thomas Gervais (Polytechnique Montréal) a développé une technologie, les afficheurs microfluidiques, qui pourrait pallier ce problème. Ces dispositifs de microfluidique ouverte permettent le contrôle spatio-temporel précis de fluides et la livraison de réactifs sur une surface ouverte, telle un plat de Pétri. L’objectif principal de ce projet est de démontrer la pertinence des afficheurs microfluidiques pour l’étude de la dynamique des voies de signalisation. ABSTRACT: Cell signaling is the fundamental biological process by which cells communicate with each other and their environment through the interaction of different signals. Cell response varies depending on the nature and intensity of a signal, but it is now well known that its temporal profile also plays an important role. Notably, the Notch signaling pathway, important in development and maintaining homeostasis, can lead to different cellular responses depending on its activation dynamics. Thus, the study of cell signaling dynamics requires precise temporal resolution of signal delivery to cells. However, traditional techniques in cell biology focused on manual reagent pipetting do not allow this resolution to be achieved. Pr Thomas Gervais’s research group (Polytechnique Montreal) has developed a technology, the microfluidic display, that could overcome this challenge. These open-space microfluidic devices provide precise spatiotemporal control of fluids and enable delivery of reagents to an open surface, such as a culture dish. The main objective of this project is to demonstrate the potential of microfluidic displays for the study of cell signaling dynamics.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".